A202 INVESTIGATION OF THE DIRECT EFFECT OF HEMIN-HEMOPEXIN AND HAPTOGLOBIN STIMULATION IN INFLAMMATORY BOWEL DISEASE

Abstract Background Inflammatory bowel disease (IBD) is a global health issue defined by persistent intestinal epithelial inflammation resulting in bleeding, and it one of the major risk factors colorectal cancer (CRC). Hemin an activator heme-oxygenase has anti-inflammatory, cytoprotective, antiapoptotic properties stimulating hemopexin haptoglobin production. Hemopexin are scavenging proteins with high affinity for hemin hemoglobin, activated interleukin (IL)-22. Purpose To evaluate protective role through stimulation experimental acute colitis. Method Mice were preconditioned intraperitoneal injection 5mg/kg body weight three days while control mice treated PBS. Colitis was induced giving 2.5-3% dextran sulfate sodium (DSS) drinking water 12 days. The activity index (DAI) determined daily monitoring weight, fecal bleeding stool consistency. At end experiment, liver, feces, serum colon samples harvested evaluation levels hemopexin, haptoglobin, IL-22, IL-22BP, IL-6 TNF-α. Fecal microbiota composition will be using 16S rRNA sequencing. Result(s) We found that treatment resulted presence samples, where they may exert influence growth pathobionts able to acquire iron heme hemoglobin as sources. Conclusion(s) findings from this study contribute understanding possible ulcerative Please acknowledge all funding agencies checking applicable boxes below CIHR Disclosure Interest None Declared